| Species |
Human |
| Protein Construction |
MICA (Glu24-Gln308)_x000D_
Accession # Q96QC4 |
hFc |
| N-term |
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized Human NKG2D, His Tag at 5μg/ml (100μl/Well) on the plate can bind MICA hFc Chimera, Human. Test result was comparable to standard batch |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
59.64 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 75-85 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig‑like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin. |
| Synonyms |
FLJ60820; MGC111087; MICA; PERB11.1; DAMA-345G11.2; FLJ36918; FLJ60820; MGC21250 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
