| Species |
Human |
| Protein Construction |
| His |
PAH (D415N) (Met1-Lys452 (D415N))_x000D_
Accession # P00439 |
| N-term |
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Expression System |
Baculovirus-Insect Cells |
| Theoretical Molecular Weight |
52.82 kDa |
| Apparent Molecular Weight |
The protein has a predicted MW of 52.82 kDa same as Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in 20mM Tris, 0.5M NaCl (pH 8.0). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Phenylalanine hydroxylase (PAH) is a member of aromatic amino acid hydroxylase (AAAHs) family, and catalyze phenylalanine (Phe) into tyrosine (Tyr). PAH is also an allosteric enzyme that maintains phenylalanine (Phe) below neurotoxic levels; its failure results in phenylketonuria, an inborn error of amino acid metabolism. |
| Synonyms |
PH; PKU; PKU1; |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
