Species |
Human |
Protein Construction |
His |
PHOSPHO1 (Met1-Cys267) Accession # Q8TCT1-1 |
N-term |
C-term |
|
Purity |
> 95% as determined by BisTris PAGE > 95% as determined by HPLC |
Endotoxin Level |
Less than 1EU per μg by the LAL method. |
Expression System |
E.coli |
Theoretical Molecular Weight |
30.67 kDa |
Apparent Molecular Weight |
The protein has a predicted MW of 30.67 kDa same as Bis-Tris PAGE result. |
Formulation |
Lyophilized from 0.22μm filtered solution in PBS, 300mM L-Arginine (pH 7.0). |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Target Background |
PHOSPHO1, a phosphoethanolamine/phosphocholine phosphatase, is upregulated in mineralising cells and is thought to be involved in the generation of inorganic phosphate for bone mineralisation. |
Synonyms |
Phosphocholine phosphatase-1 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.