| Species |
Human |
| Protein Construction |
PODXL2 (Gly33-Thr500)_x000D_
Accession # Q9NZ53 |
His |
| N-term |
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1 EU per μg by the LAL method. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
51.5 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 85-115 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Transmembrane protein endoglycan (PODXL2), which belongs to the CD34-family of highly glycosylated sialomucins. Endoglycan is broadly expressed in the developing mouse brains and is proteolytically shed in vitro in mouse neurons and in vivo in mouse brains. Endoglycan shedding in primary neurons was mediated by the transmembrane protease a disintegrin and metalloprotease 10 (ADAM10), but not by its homolog ADAM17. |
| Synonyms |
Endoglycan; PODLX2; brain-1; BRN1; EG; oct-8; OTF8; UNQ1861/PRO3742 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
