| Species |
Human |
| Protein Construction |
Siglec-10 (R119A) (Met17-Thr546)_x000D_
Accession # Q96LC7-1 |
His |
Avi |
| N-term |
|
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per ug by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized Siglec-10 (R119A), His & Avi, Human at 1μg/ml (100μl/well) on the plate can bind AntiSiglec10 (R119A) Antibody, hFc Tag. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
62.64 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 65-80 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in 20mM Tris,150mM NaCl, 200mM Arginine (pH 8.0). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system. |
| Synonyms |
SLG2; SIGLEC10; MGC126774; PRO940 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
