| Species |
Human |
| Protein Construction |
Siglec-2/CD22 (Asp20-Arg687)
Accession # P20273-1 |
His |
| N-term |
C-term |
|
| Purity |
> 95% as determined by Bis-Tris PAGE |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized Siglec-2/CD22, His, Human at 1μg/ml (100μl/well) on the plate can bind Anti-Siglec-2 Antibody, hFc Tag. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
76.2 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 100-120 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from a 0.22 μm filtered solution in PBS, (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized Siglec-2/CD22, His, Human, His Tag at 1 μg/ml (100 μL/well) on the plate. Dose response curve for Anti-Siglec-2 Antibody, hFc Tag with the EC50 of 6.6 ng/ml determined by ELISA.
Siglec-2/CD22, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
| Target Background |
CD22, or cluster of differentiation-22, is a molecule belonging to the SIGLEC family of lectins. It is found on the surface of mature B cells and to a lesser extent on some immature B cells. CD22 a member of the immunoglobulin superfamily. CD22 functions as an inhibitory receptor for B cell receptor (BCR) signaling. It is also involved in the B cell trafficking to Peyer's patches in mice. |
| Synonyms |
CD22 molecule; CD22; Siglec-2; B-cell receptor CD22; BL-CAM; SIGLEC2; SIGLEC2FLJ22814 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
