Species |
Human |
Protein Construction |
Sonic Hedgehog (Shh) (Cys24-Gly197)_x000D_ Accession # Q15465 |
|
Purity |
> 95% as determined by BisTris PAGE > 95% as determined by HPLC |
Endotoxin Level |
Less than 0.05EU per μg by the LAL method. |
Expression System |
E.coli |
Theoretical Molecular Weight |
19.79 kDa kDa |
Apparent Molecular Weight |
The protein migrates to 23-25 kDa based on Bis-Tris PAGE result. |
Formulation |
Lyophilized from 0.22 μm filtered solution in PBS, 300mM NaCl (pH 7.4). |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Target Background |
Sonic hedgehog (Shh) is a secreted protein with important roles in mammalian embryogenesis. During tooth development, Shh is primarily expressed in the dental epithelium, from initiation to the root formation stages. |
Synonyms |
SHH; Sonic Hedgehog; HHG-1; Sonic hedgehog; HLP3; HPE3; SMMCI; TPT; TPTPS |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.