| Species |
Human/Mouse/Rat |
| Protein Construction |
GDF-8 (Asp267-Ser375)_x000D_
Accession # O14793(Human) / O08689(Mouse) / O35312(Rat) |
|
| Purity |
> 95% as determined by Bis-Tris PAGE |
| Endotoxin Level |
Less than 1 EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized GDF-8, Human/Mouse/Rat at 2μg/ml (100 μl/well) on the plate can bind AntiGDF8 Antibody, hFc Tag. Test result was comparable to standard batch.
Determined by its ability to inhibit the proliferation of MPC11 cells. The expected ED50 for this effect is < 30 ng/ml. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
12.40 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 13-15 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in 4 mM HCl. |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 4 mM HCl. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Growth/differentiation factor 8 (GDF8), or myostatin, negatively regulates muscle mass. GDF8 is held in a latent state through interactions with its N-terminal prodomain. GDF8, like numerous TGF-β family members, is a disulfidelinked dimer that is synthesized as a precursor protein which requires cleavage by a furin-like protease to yield an N-terminal prodomain and a C-terminal mature, signaling domain. |
| Synonyms |
Myostatin; GDF8; Mstn; GDF-8; Myostatin |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
