| Species |
Human/Mouse/Rat |
| Protein Construction |
BDNF (His129-Arg247(Human) / His131-Arg249(Mouse/Rat))_x000D_
Accession # P23560-1(Human) / P21237-1(Mouse) / P23363(Rat) |
|
| Purity |
> 95% as determined by Bis-Tris PAGE |
| Endotoxin Level |
Less than 0.05 EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized BDNF, Human/Mouse/Rat at 1μg/ml (100 μl/well) on the plate can bind Human TrkB, His Tag. Test result was comparable to standard batch. |
| Expression System |
E.coli |
| Theoretical Molecular Weight |
13.51 kDa |
| Apparent Molecular Weight |
The protein migrates to 15-19 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
The brain-derived neurotrophic factor (BDNF) is a secretory growth factor that promotes neuronal proliferation and survival, synaptic plasticity and long-term potentiation in the central nervous system. Brain-derived neurotrophic factor biosynthesis and secretion are chrono-topically regulated processes at the cellular level, accounting for specific localizations and functions. |
| Synonyms |
Abrineurin; BDNF; ANON2; BULN2 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
