| Species |
Monkeypox virus |
| Protein Construction |
| His |
E8L (Met1-Thr275)
Accession # QJQ40248.1 |
| N-term |
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Expression System |
E.coli |
| Theoretical Molecular Weight |
32.92 kDa |
| Apparent Molecular Weight |
The protein has a predicted MW of 32.92 kDa. The protein migrates to 50-60 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
E8L is an important protein that mediates the invasion of monkeypox virus into host cells. In the process of invading and completing replication, E8L acts as a surface-binding protein of mature virion and can bind to chondroitin sulfate on the cell surface, so that the virus can attach to target cells. |
| Synonyms |
Cell surface-binding protein; Carbonic anhydrase homolog |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
