| Species |
Mouse |
| Protein Construction |
Activin RIIA/ACVR2A (Ala20-Pro135)_x000D_
Accession # P27038 |
His |
| N-term |
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1 EU per μg by the LAL method. |
| Biological Activity |
Activin RIIA/ACVR2A, His, Mouse captured on CM5 Chip via AntiHis Antibody can bind Human Activin A, No Tag in SPR assay (Biacore T200). Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
15.06 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Activin A receptor type 2A (ACVR2A) is a membrane receptor in the transforming growth factor- beta (TGF-β signaling pathway, which is involved in the regulation of cell proliferation, migration, and apoptosis. Loss of ACVR2A has an important role in cancer progression and distant metastasis and may serve as a prognostic marker in patients with colon cancer. |
| Synonyms |
ACTR-IIA; ACTRIIA; ACVR2A; ACVR2 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
