| Species |
Mouse |
| Protein Construction |
Beta Klotho (Phe53-Pro994)_x000D_
Accession # Q99N32-1 |
His |
| N-term |
C-term |
|
| Purity |
> 90% as determined by Bis-Tris PAGE
> 90% as determined by HPLC |
| Endotoxin Level |
Less than 1 EU per μg by the LAL method. |
| Biological Activity |
Human FGFR4, hFc Tag captured on CM5 Chip via Protein A can bind Beta Klotho, His, Mouse in SPR assay (Biacore T200). Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
109.91 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 120-130 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Beta-klotho (KLB) is a coreceptor required for endocrine fibroblast growth factor (FGF) 15/19 and FGF21 signaling in the brain. Klb is prominent within the hypothalamus, which is consistent with its metabolic functions, but diverse roles for Klb are now emerging. Central Klb expression is low but discrete and may govern FGF-targeted sites. |
| Synonyms |
betaKlotho; beta-klotho; BKL; KLB; klotho beta like; Klotho beta; MGC142213;βKlotho; β-klotho; klotho β like; Klotho β |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
