| Species |
Mouse |
| Protein Construction |
FcRn [Ser22-Ser297 (FCGRT)&Ile21-Met119 (B2M)]
Accession # Q61559(FCGRT)&P01887(B2M) |
His |
| N-term |
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1 EU per μg by the LAL method. |
| Biological Activity |
FcRn, His, Mouse captured on CM5 Chip via antihis antibody can bind Trastuzumab in SPR assay (Biacore T200). Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
32.1 kDa (FCGRT)&11.7 kDa (B2M) |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 50-55 kDa (FCGRT)&14-15 kDa (B2M) based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells. FCGRT&B2M heterodimer protein (FcRn complex) consist of two subunits: p51, and p14 , and forms an MHC class I-like heterodimer. |
| Synonyms |
FcRn alpha chain; FCGRT; FCRN; FCGRT&B2M |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
