| Species |
Mouse |
| Protein Construction |
G-CSF R/CD114 (Cys26-Asp626)_x000D_
Accession # P40223 |
His |
| N-term |
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized G-CSF R/CD114, His, Mouse at 0.2μg/ml (100μl/well) on the plate can bind Human GCSF, hFc Tag. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
68.47 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
The Granulocyte Colony-Stimulating Factor (G-CSF) receptor, a member of the hematopoietin cytokine receptor superfamily, functions as a homodimer and requires the recruitment of cytosolic protein tyrosine kinases (PTKs) to transduce its signal. |
| Synonyms |
G-CSF-R; G-CSF R; CD114; G CSF R; CSF3R; Csfgr; GCSFR |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
