| Species |
Mouse |
| Protein Construction |
GFRA3 (Asn29-Arg379)_x000D_
Accession # O35118 |
His |
| N-term |
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized Mouse ARTN, hFc Tag at 5μg/ml (100μl/well) on the plate can bind GFRA3, His, Mouse. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
40.3 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 47-52 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
GDNF (glial-cell-line derived neurotrophic factor) is a potent neurotrophic factor for dopaminergic neurons. Neuropsychiatric diseases and their treatments are associated with alterations in the levels of both GDNF and its receptor family (GDNF family receptor alpha or GFRA). GFRA1, GFRA2 and GFRA3 are located in chromosomal regions with suggestive linkage to schizophrenia. |
| Synonyms |
GDNFR-alpha-3; GFR-alpha-3; GFRA3; GFR alpha3; GPI-linked receptor |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
