| Species |
Mouse |
| Protein Construction |
IgG2a Fc (Glu98-Lys330)_x000D_
Accession # P01863 |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Mouse FcRn, His Tag captured on CM5 Chip via AntiHis Antibody can bind IgG2a Fc, Mouse in SPR assay (Biacore T200). Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
26.38 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 33-38 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Mouse IgG2a is one of the subtypes of murine immunoglobulin IgG which mediates immune responses to carbohydrate or polysaccharide antigens. The Fc region of IgG2a can bind to FcRn to prolong its half-life. |
| Synonyms |
IgG2a; IgG2a-Fc; Igh-1; Igh 1; Immunoglobulin G2A Fc; G2A Fc |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
