| Species |
Mouse |
| Protein Construction |
IL-12 [Arg23-Ala215(IL-12A)&Met23-Ser335(IL-12B)]
Accession # P43431(IL-12A)&P43432(IL-12B) |
His |
| N-term |
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK92 cells. The ED50 for this effect is 0.5 2 ng/mL. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
22.80 kDa(IL-12A)&35.79 kDa(IL-12B) |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 25-30 kDa(IL-12A)&45-55 kDa(IL-12B) based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably. |
| Synonyms |
CLMF; CLMF2; IL-12A; IL-12B; IL12; IL12 p70; IMD28; IMD29; NFSK; NKSF1; NKSF2; P35; IL-12 subunit p35; IL12A; interleukin 12 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
