| Species |
Mouse |
| Protein Construction |
IL-3 (Ala27-Cys166)_x000D_
Accession # P01586 |
His |
| N-term |
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured in a cell proliferation assay using NFS60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 0.1 0.5 ng/mL. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
16.77 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 27-40 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes. |
| Synonyms |
IL3; IL-3; IL-3MGC79398; interleukin-3; MULTI-CSF; MCGF |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
