| Species |
Mouse |
| Protein Construction |
Integrin alpha 2 beta 1 (ITGA2&ITGB1) Heterodimer[Tyr27-Thr1129(ITGA2) acidic tail & Gln21-Asp728(ITGB1) basic tail]
Accession # Q62469(ITGA2)&P09055-1(ITGB1) |
His |
| N-term |
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
126.60 kDa(ITGA2)&82.80 kDa(ITGB1) |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 128-138 kDa(ITGA2)&105-115 kDa(ITGB1) based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Integrin alpha 2 beta 1 is a positive regulator of collagenase (MMP-1) and collagen alpha 1(I) gene expression.HOS cells normally lacking alpha 2 beta 1 integrin were forced to express it, and this prevented the down-regulation in the levels of alpha 1 (I) collagen mRNA when cells were grown inside collagen gels. The data indicate that the level of MMP-1 expression is regulated by the collagen receptor alpha 2 beta 1 integrin. |
| Synonyms |
α2β1; ITGA2&ITGB1; ITGA2; ITGB1; |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
