| Species |
Mouse |
| Protein Construction |
P4HB (Asp20-Leu509)_x000D_
Accession # P09103 |
His |
| N-term |
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its ability to promote aggregation of insulin in the presence of DTT. The specific activity is > 10 A650/cm/min/mg. Test result meets the standard. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
56.18 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 57-70 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Protein disulfide isomerase (PDI, also named P4HB) is a 57-kDa oxidoreductase that facilitates cysteine thiol reactions inside and outside the cell. It mediates reduction or oxidation of protein disulfide bonds, thiol/disulfide exchange reactions, and transfer of NO from one protein thiol to another. It also has chaperone properties. PDI is actively secreted by most, if not all, of the cell types involved in thrombosis, binds to integrins on the cell surface, and circulates as a soluble protein in blood. |
| Synonyms |
PDI; ERp59; p55; Pdia1; Thbp |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
