| Species |
Mouse |
| Protein Construction |
R spondin 1/RSPO1 (Ser21-Gln265)_x000D_
Accession # Q9Z132 |
His |
| N-term |
C-term |
|
| Purity |
> 95% as determined by Bis-Tris PAGE |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized R spondin 1/RSPO1, His, Mouse at 5μg/ml (100μl/Well) on the plate can bind Mouse RNF43, hFc Tag. Test result was comparable to standard batch. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 2 20 ng/ml in the presence of 5 ng/mL recombinant Human Wnt SurrogateFc Fusion (WNTHM23A).Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
28.3 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 45-48 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in 20mM MES, 150mM NaCl, 200mM Arginine (pH 5.5). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM MES, 150mM NaCl, 200mM Arginine (pH 5.5). |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated 'thrombin-sensitive protein. |
| Synonyms |
R-spondin-1; RSPO1; CRISTIN3; FLJ40906; RP11-566C13.1; RSPO; R-Spondin1 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
