| Species |
Mouse |
| Protein Construction |
VCAM-1/CD106 (Phe25-Glu698)_x000D_
Accession # NP_035823.3 |
His |
| N-term |
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by the ability of the immobilized protein to support adhesion of U937 human histiocytic lymphoma cells. When 5 x 104 cells/well are added to VCAM-1/CD106, His, Mouse coated plates (10 μg/ml with 100 μl/well) , approximately 70% 80 % cells will adhere after 1 hour incubation at room temperature. Test result meets the standard. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
75.48 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 80-100 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Tumor necrosis factor alpha (TNFα) is a pro-inflammatory cytokine that triggers the expression of inflammatory molecules, including other cytokines and cell adhesion molecules. TNFα induces the expression of intercellular cell adhesion molecule-1 and vascular cell adhesion molecule-1 (VCAM-1). VCAM-1 was originally identified as a cell adhesion molecule that helps regulate inflammation-associated vascular adhesion and the transendothelial migration of leukocytes, such as macrophages and T cells. |
| Synonyms |
V-CAM 1; VCAM-1; INCAM-100; CD106; DKFZp779G2333; MGC99561 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
