Species |
Rat |
Protein Construction |
His |
CKMT2 (Asp40-Lys419)_x000D_ Accession # NP_001121124.1 |
N-term |
C-term |
|
Purity |
> 95% as determined by BisTris PAGE > 95% as determined by HPLC |
Endotoxin Level |
Less than 1EU per μg by the LAL method. |
Expression System |
E.coli |
Theoretical Molecular Weight |
44.48 kDa |
Apparent Molecular Weight |
The protein has a predicted MW of 44.48 kDa same as Bis-Tris PAGE result. |
Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Target Background |
Mitochondrial Creatine Kinase (MtCK) is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine, and exists in mammals as two isoenzymes encoded by separate genes. In rats and humans, sarcomere-specific MtCK (sMtCK) is expressed only in skeletal and heart muscle, and has 87% nucleotide identity across the 1257 bp coding region. |
Synonyms |
Mib-CK; Mib CK; S-MtCK; S MtCK; |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.