| Species |
Human |
| Protein Construction |
HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer [Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and KVLEYVIKV peptide]
Accession # A0A140T913(HLA-A*02:01)&P61769(B2M)&KVLEYVIKV |
His |
Avi |
| N-term |
|
C-term |
|
| Conjugate |
Biotin |
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer[Biotin], His & Avi, Human at 0.5μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate can bind Anti-B2M Antibody, mFc Tag. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
50.50 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 53-65 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
![HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer[Biotin], His & Avi, Human](/product/documents/down?doc_name=Z06469 ELISA.jpg&file=scm_files/productFile_notes/2025/04/25/Z06469 ELISA.jpg "HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer[Biotin], His & Avi, Human")
Immobilized HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer[Biotin], His & Avi, Human, His Tag at 0.5 μg/ml (100 μl/Well) on streptavidin(5 μg/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 6.8 ng/ml determined by ELISA.
![HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer[Biotin], His & Avi, Human](/product/documents/down?doc_name=Z06469 HPLC.png&file=scm_files/productFile_notes/2025/04/25/Z06469 HPLC.png "HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer[Biotin], His & Avi, Human")
The purity of HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.
![HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer[Biotin], His & Avi, Human](/product/documents/down?doc_name=Z06469 PAGE.png&file=scm_files/productFile_notes/2025/04/25/Z06469 PAGE.png "HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer[Biotin], His & Avi, Human")
HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
| Target Background |
MAGE-A1 belongs to the chromosome X-clustered genes of cancer-testis antigen family and is normally expressed in the human germ line but is also overexpressed in various tumors. |
| Synonyms |
MAGE-A1; MAGE A1; CT1.1; MAGE-1 antigen; MAGE-1; MZ2-E; MAGE1; MAGE1A |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
