Species |
Human |
Protein Construction |
HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer [Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and FMNKFIYEI peptide] Accession # A0A140T913(HLA-A*02:01)&P61769(B2M)&FMNKFIYEI |
His |
Avi |
N-term |
|
C-term |
|
Purity |
> 95% as determined by BisTris PAGE > 95% as determined by HPLC |
Endotoxin Level |
Less than 1EU per μg by the LAL method. |
Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer, His & Avi, Human at 1μg/ml (100μl/well) on the plate can bind AntiHLAA*02:01&B2M&AFP (FMNKFIYEI) Antibody, hFc Tag. Test result was comparable to standard batch. |
Expression System |
HEK293 |
Theoretical Molecular Weight |
50.6 kDa |
Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 53-60 kDa based on Bis-Tris PAGE result. |
Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |

Immobilized HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer, His & Avi, Human, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&AFP Antibody, hFc Tag with the EC50 of 1.9 ng/ml determined by ELISA.

The purity of HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer, His & Avi, Human is greater than 95% as determined by SEC-HPLC.

HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Target Background |
Alpha-fetoprotein (AFP), a specific liver cancer marker, T cells expressing AFP-CAR selectively degranulated, released cytokines, and lysed liver cancer cells that were HLA-A*02:01 /AFP while sparing cells from multiple tissue types that were negative for either expressed proteins.CAR T-cell immunotherapy targeting intracellular/secreted solid tumor antigens can elicit a potent antitumor response. |
Synonyms |
Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-feto; AFP; HPAFP; AFPD; FETA; MHC |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.