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Large-Scale Antibody Services> | ...All MOs or mRNAs were injected at the one-cell stage as de- scribed previously [21]. Antibody production and Western blot A peptide (CVSEVESGELRWPPE) was synthesized and conjugated to the KLH peptide (<b>genscript</b> Company).... | Get A Quote |
The homeobox transcription factor orthodenticle homolog 2 (otx2) is supposed as an organizer that orchestrates a transcription factor network during photoreceptor development. However, its regulation in the process remains unclear. In this study, we have identified a zebrafish limb bud and heart-like gene (lbh-like), which is expressed initially at 30 hours post fertilization (hpf) in the developing brain and eyes. Lbh-like knockdown by morpholinos specifically inhibits expression of multiple photoreceptor-specific genes, such as opsins, gnat1, gnat2 and irbp. Interestingly, otx2 expression in the morphants is not significantly reduced until 32 hpf when lbh-like begins to express, but its expression level in 72... More