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The plant defensin NaD1 introduces membrane disorder through a specific interaction with the lipid, phosphatidylinositol 4, 5 bisphosphate.

Biochim Biophys Acta.. 2016-06; 
Payne JA, Bleackley MR, Lee TH, Shafee TM, Poon IK, Hulett MD, Aguilar MI, van der Weerden NL, Anderson MA.
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Catalog Products ... 2. Experimental Procedures. 2.1. Protein sources. The defensins NaD1 and NaD2 were purified from the flowers of N. alata as described in Dracatos et al. [21] and Lay et al. [22]. CP29 and Histatin 5 were purchased from GenScript (Hong Kong China). ... Get A Quote

摘要

Plant defensins interact with phospholipids in bilayers as part of their cytotoxic activity. Solanaceous class II defensins with the loop 5 sequence pattern "S-[KR]-[ILVQ]-[ILVQ]-[KR]-[KR]" interact with PI(4,5)P2. Here, the prototypical defensin of this class, NaD1, is used to characterise the biophysical interactions between these defensins and phospholipid bilayers. Binding of NaD1 to bilayers containing PI(4,5)P2 occurs rapidly and the interaction is very strong. Dual polarisation interferometry revealed that NaD1 does not dissociate from bilayers containing PI(4,5)P2. Binding of NaD1 to bilayers with or without PI(4,5)P2 induced disorder in the bilayer. However, permeabilisation assays revealed that NaD1 o... More

关键词

Kinetics; Membrane disorder; Membrane fusion; Phosphatidylinositol 4,5 bisphosphate; Plant defensin; Protein–lipid interaction