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Cloning and expression of long neuropeptide F and the role of FMRFamide-like peptides in regulating egg production in the Chagas vector, Rhodnius prolixus.

Peptides.. 2016-08; 
Sedra L, Lange AB.
Products/Services Used Details Operation
Catalog Products ... GNDNFMRFamide, AKDNFIRFamide, the sulfakinin RhoprSK-1 (pQFNEY(SO 3 )GHMRFamide), the myosuppressin RhoprMS (pQDIDHVFMRFamide) and the allatostatin RhoprAST-2 (LPVYNFGLamide) were purchased as powders from GenScript (Piscataway, NJ, USA). ... Get A Quote

摘要

Long neuropeptide F (NPF) is a neuropeptide implicated in the control of feeding, digestion and reproduction in various insect species. Here we have isolated the cDNA sequence encoding NPF in Rhodnius prolixus (RhoprNPF). The RhoprNPF gene is composed of 3 exons and 2 introns, one of which is present in the peptide coding region. RhoprNPF is 42 amino acids long and has the characteristic RFamide C-terminus, which is common of FMRFamide-like peptides (FLPs). Quantitative PCR (qPCR) shows that RhoprNPF mRNA is present in higher amounts in fifth instars than in adults, implying that it may play a role in growth and development. In situ hybridization shows that the RhoprNPF transcript is present in median neurosecr... More

关键词

Egg-laying; In situ hybridization; Insect; Neuropeptide; Ovulation