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Gradually softening hydrogels for modeling hepatic stellate cell behavior during fibrosis regression.

Integr Biol (Camb).. 2016-06; 
Caliari SR, Perepelyuk M, Soulas EM, Lee GY, Wells RG, Burdick JA.
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Catalog Products ... by 1 H NMR (Bruker). MeHA was further modified with 1 mM thiolated RGD (GCGYGRGD- SPG, GenScript) via Michael-type addition (45 min, room temperature, pH 9) to permit cell ad- hesion. 2.2 MeHA hydrogel fabrication. ... Get A Quote

摘要

The extracellular matrix (ECM) presents an evolving set of mechanical cues to resident cells. We developed methacrylated hyaluronic acid (MeHA) hydrogels containing both stable and hydrolytically degradable crosslinks to provide cells with a gradually softening (but not fully degradable) milieu, mimicking physiological events such as fibrosis regression. To demonstrate the utility of this cell culture system, we studied the phenotype of rat hepatic stellate cells, the major liver precursors of fibrogenic myofibroblasts, within this softening environment. Stellate cells that were mechanically primed on tissue culture plastic attained a myofibroblast phenotype, which persisted when seeded onto stiff (∼20 kPa) h... More

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