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The periplasmic sensing domain of Vibrio fischeri chemoreceptor protein A (VfcA): cloning, purification and crystallographic analysis.

Acta Crystallogr F Struct Biol Commun.. 2016-05;

Salah Ud-Din AI, Roujeinikova A.

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PCR Cloning and Subcloning	... 305, 567-580.] ; Fig. 1 [link] ). The Escherichia coli codon-optimized DNA fragment encoding the periplasmic sensory domain of VfcA (VfcA peri ; amino-acid residues 26–272) was synthesized and ligated into the expression vector pET151/D-TOPO (Invitrogen) by GenScript. ...	Get A Quote

摘要

Flagella-mediated motility and chemotaxis towards nutrients are important characteristics of Vibrio fischeri that play a crucial role in the development of its symbiotic relationship with its Hawaiian squid host Euprymna scolopes. The V. fischeri chemoreceptor A (VfcA) mediates chemotaxis toward amino acids. The periplasmic sensory domain of VfcA has been crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol 3350 as a precipitating agent. The crystals belonged to space group P1, with unit-cell parameters a = 39.9, b = 57.0, c = 117.0 Å, α = 88.9, β = 80.5, γ = 89.7°. A complete X-ray diffraction data set has been collected to 1.8 Å resolution using cryocooling conditions... More