| Products/Services Used | Details | Operation |
|---|---|---|
| PCR Cloning and Subcloning | ... oligonucleotides with targeting sequences to MALAT1 were designed as follows: 5′-GATCCGGAGGTGTAAAGGGATTTATTTTTCAAGAGAAAATAAATCCCTTTACACCTCTTTTTTG- 3′. The oligonucleotides were cloned into the pLVX-shRNA1 vector (GenScript, Piscataway, ... | Get A Quote |
BACKGROUND: MALAT1 is recognized as an oncogenic lncRNA in various malignancies. However, its expression and function in oral tongue squamous cell carcinoma are still unknown. This study aims to investigate the expression and function of MALAT1 in TSCC tissues and cells. MATERIALS AND METHODS: qPCR was performed to detect the expression of MALAT1. MALAT1 was suppressed and upregulated by plasmid transfection in TSCC cells, and then cell migration, invasion, EMT, and apoptosis were analyzed. RESULTS: LncRNA MALAT1 was upregulated in TSCC tissues and correlated with cervical lymph node metastasis in TSCC patients. Moreover, MALAT1 induced cell migration, invasion, EMT, and inhibited apoptosis by modulating Wnt... More
