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Bacterial Protein Expression System | ... previously [38]. UGDH and NOX were stored at ‐20°C and 4°C, respectively. The gene of GalK from E. coli K12 (X02306.1) [39] [40] was ordered from GenScript USA Inc. (Piscataway, NJ, USA) with the restrictions sides NdeI and BamHI (Figure S29). With ... | Get A Quote |
Nucleotide sugars are considered as bottleneck and expensive substrates for enzymatic glycan synthesis using Leloir-glycosyltransferases. Synthesis from cheap substrates such as monosaccharides is accomplished by multi-enzyme cascade reactions. Optimization of product yields in such enzyme modules is dependent on the interplay of multiple parameters of the individual enzymes and governed by a considerable time effort when convential analytic methods like capillary electrophoresis (CE) or HPLC are applied. We here demonstrate for the first time multiplexed CE (MP-CE) as fast analytical tool for the optimization of nucleotide sugar synthesis with multi-enzyme cascade reactions. We introduce a universal separation... More