至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Multiplexed Capillary Electrophoresis as Analytical Tool for Fast Optimization of Multi‐Enzyme Cascade Reactions–Synthesis of Nucleotide Sugars.

Biotechnol J.. 2016-10; 
C Wahl, D Hirtz, L Elling.
Products/Services Used Details Operation
Bacterial Protein Expression System ... previously [38]. UGDH and NOX were stored at ‐20°C and 4°C, respectively. The gene of GalK from E. coli K12 (X02306.1) [39] [40] was ordered from GenScript USA Inc. (Piscataway, NJ, USA) with the restrictions sides NdeI and BamHI (Figure S29). With ... Get A Quote

摘要

Nucleotide sugars are considered as bottleneck and expensive substrates for enzymatic glycan synthesis using Leloir-glycosyltransferases. Synthesis from cheap substrates such as monosaccharides is accomplished by multi-enzyme cascade reactions. Optimization of product yields in such enzyme modules is dependent on the interplay of multiple parameters of the individual enzymes and governed by a considerable time effort when convential analytic methods like capillary electrophoresis (CE) or HPLC are applied. We here demonstrate for the first time multiplexed CE (MP-CE) as fast analytical tool for the optimization of nucleotide sugar synthesis with multi-enzyme cascade reactions. We introduce a universal separation... More

关键词

Analytical biotechnology; Biocatalysis; Multi-enzyme cascade; Nucleotide sugar; Synthetic biology