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Bioinformatics Tools> | ... Primer sequences (Table 1) were designed based on the Zebrafish mRNA sequences available in the DDBJ/Gen Bank TM/EBI data bank using the Primer 3 software (http://frodo.wi.mit.edu/primer3) obtained from GenScript, United States. ... | Get A Quote |
Studies on transcriptional modulation after gamma radiation exposure in fish are limited. Cell cycle perturbations and expression of apoptotic genes were investigated in the fish, Catla catla after acute and protracted exposures to gamma radiation over a 90day period. Significant changes in gene expression were observed between day 1 and 90 post-exposure. Gamma radiation induced a significant down-regulation of target genes gadd45α, cdk1 and bcl-2 from day 1 to day 3 after protracted exposure, whereas it persists till day 6 upon acute exposure. From day 12 onwards, Gadd45α, cdk1 and bcl-2 genes were up-regulated following protracted exposure, indicating DNA repair, cell-cycle arrest and apoptosis. There exist... More