至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Functional analysis of PCSK2 coding variants: a founder effect in the Old Order Amish population.

Diabetes Res Clin Pract.. 2017-09; 
Winters A,Ramos-Molina B,Jarvela TS,Yerges-Armstrong L,Pollin TI,Lindberg I.
Products/Services Used Details Operation
Mutagenesis Services ... Mutagenesis was performed by GenScript (Piscataway, NJ); all constructs were verified by full insert sequencing. A eukaryotic expression plasmid encoding human proglucagon was obtained from FivePrime Therapeutics (San Francisco, CA). 2.6. Cell culture. ... Get A Quote

摘要

AIMS: In humans, noncoding variants of PCSK2, the gene encoding prohormone convertase 2 (PC2), have been previously associated with risk for and age of onset of type 2 diabetes (T2D). The aims of this study were to identify coding variants in PCSK2; to determine their possible association with glucose handling; and to determine functional outcomes for coding variants in biochemical studies. METHODS: Exome-wide genotyping was performed on 1725 Old Order Amish (OOA) subjects. PCSK2 coding variants were tested for association with diabetes-related phenotypes. In vitro analyses using transfected human PC2-encoding constructs were performed to determine the impact of each mutation on PC2 activity. RESULTS: We ide... More

关键词

Diabetes; Glucagon; Glucose; PCSK2; Prohormone convertase 2