| Products/Services Used | Details | Operation |
|---|---|---|
| PCR Cloning and Subcloning | ... 13. PUC57-rab.GP (codon-optimized for improving expression, Genscript). 14. pShuttle (as described in Ref. ... 1). 1. Cloning the rab.GP gene into pShuttle. Digest 500 ng of PUC57-rab.GP (Genscript) and 500 ng of pShuttle [17] with XbaI and NheI for 2 h at 37 °C, respectively. ... | Get A Quote |
| Bacterial Expression System | Get A Quote |
Vaccination is considered to be the most effective method of preventing infectious or other diseases. Adenovirus (Ad) is one the most promising vectors in vaccine research and development. It can induce not only potent humoral but also cellular immune responses, and has therefore been widely applied in basic and translational studies. Chimpanzee Ad is a rare serotype circulating in humans. This circumvents the problem of preexisting immunity to human Ad serotypes, enhancing Chimpanzee Ad prospects in vaccine development. Here we describe experimental procedures used to generate a new generation of rabies vaccine based on a chimpanzee Ad vector, which can be extended in the development of novel vaccines against ... More
