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Development of a rapid magnetic bead-based immunoassay for sensitive detection of zearalenone.

Food Control.. 2017-09; 
F Zhao,Q Shen,H Wang,X Han,Z Yang.
Products/Services Used Details Operation
Catalog Products ... (St. Louis, MO, USA). Carboxyl modified polystyrene magnetic microspheres (diameter 0.5–1 μm) were purchased from BaseLine Chromtech Research Centre (Tianjin, China). Protein G resin was purchased from GenScript Biotechnology Co., Ltd (Nanjing, Jiangsu, China). ... Get A Quote

摘要

Here we demonstrate a novel magnetic bead-based enzyme-linked immunosorbent assay (MB-ELISA) for zearalenone (ZEN) detection. Firstly, an anti-ZEN monoclonal antibody (mAb) was prepared by hybridoma technique, and immobilized on carboxyl modified MBs to obtain mAb-MBs. In addition, the biotinylated ZEN-BSA was labelled by streptavidin-HRP for use as competitor. Based on the mAb-MBs and streptavidin-HRP labelled ZEN-BSA, a MB-ELISA which contains only one 20 min antigen-antibody reaction step and takes no more than 45 min for dozens of samples analysis was developed. The half maximal inhibitory concentration (IC50) and limit of detection (LOD) of the MB-ELISA are 1.78 ng/mL and 0.13 ng/mL, respectively. And ... More

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