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Functional characterization of an N-terminally truncated mitochondrial porin expressed in Neurospora crassa.

Can J Microbiol.. 2017-08; 
Shuvo SR,Kovaltchouk U,Zubaer A,Kumar A,Summers WAT,Donald LJ,Hausner G,Court DA.
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Catalog Products ... Universität Page 5 of 32 Page 6. 6 München) or residues 195-210 (GenScript, Piscataway, NJ). Mitochondrial analyses Mitochondria were isolated according to Harkness et al. (1994), following growth in Vogel's Medium (VM). … Get A Quote

摘要

Mitochondrial porin, which forms voltage-dependent anion-selective channels (VDAC) in the outer membrane, can be folded into a 19-β-stranded barrel. The N terminus of the protein is external to the barrel and contains α-helical structure. Targeted modifications of the N-terminal region have been assessed in artificial membranes, leading to different models for gating in vitro. However, the in vivo requirements for gating and the N-terminal segment of porin are less well-understood. Using Neurospora crassa porin as a model, the effects of a partial deletion of the N-terminal segment were investigated. The protein, ΔN2-12porin, is assembled into the outer membrane, albeit at lower levels than the wild-type pro... More

关键词

N terminus; Neurospora crassa; VDAC; alternative oxidase; extrémité N-terminale; mitochondrial porin; oxydase alternative; porine mitochondriale