CRISPR/cas9: An Emerging Revolution in Therapeutics.

Clustered Regular Interspaced Short Palindromic Repeats also known as CRISPR, the most rapidly adopted genome editing tool was discovered through studies on bacterial immunity system. As genome editing and studies related to its functionality requires to manipulate DNA and modify genomes. Already available genome editing tools like HR-mediated targeting, ZFN, TALEN and Cre-lox etc have certain limitation but the challenge had overcome by CRISPR combined with cas9 protein. Because of the fact that future of medicinal and therapeutics seems to reside in adjustments at molecular level by genetic modifications and genome editing in cells. Alteration of a gene expression, inhibition of a gene through advanced methodologies of CRISPR/cas can provide insight to the key cause of many globally recognized diseases. This review is brief overview of highlighted present day exigent assignments of this technology in various medical and biotechnological areas to explore further horizons for therapeutic domain of research.