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Molecular Biology Tools> | ... MWs and pI values were calculated using the Compute pI/Mw tool (http://ca.expasy.org/tools/pi_tool. html). Gene-specific primers were designed using an online program (https://www.genscript.com/ ssl-bin/app/primer) (Table 1). Table 1. Primers used in this study. ... | Get A Quote |
Members of the genus Ixeris have long been used in traditional medicines as stomachics, sedatives, and diuretics. Phenylalanine ammonia-lyase (PAL), cinnamate-4-hydroxylase (C4H), 4-coumarate: coenzyme-A (CoA) ligase (4CL), chalcone synthase (CHS), and dihydroflavonol 4-reductase (DFR) are important enzymes in the phenylpropanoid pathway. In this study, we analyzed seven genes from Ixeris dentata var. albiflora that are involved in phenylpropanoid biosynthesis, using an Illumina/Solexa HiSeq 2000 platform. The amino acid sequence alignments for IdPALs, IdC4H, Id4CLs, IdCHS, and IdDFR showed high identity to sequences from other plants. We also investigated transcript levels using quantitative real-time PCR, and... More