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Bioinformatics Tools> | ... Gels were run at 100–130 V and then stained with eStain2.0 and protein staining pads (GenScript, Piscataway, NJ) according to the manufacturer's protocol. Gels were then imaged on an Odyssey CLx (LiCor Biosciences, Lincoln, NE) using Image Studio software. 2.3. ... | Get A Quote |
To develop a prophylactic for organophosphorus (OP) poisoning utilizing catalytic bioscavengers, the circulatory stability of the enzymes needs to be increased. One strategy for increasing the bioavailability of OP bioscavengers is to target them to the surface of red blood cells (RBCs). Given the circulatory lifespan of 120 days for human RBCs, this strategy has the potential for creating a persistent pool of bioscavenger. Here we report the development of fusion proteins with a single chain variable fragment (scFv) of Ter119, a molecule that associates with glycophorin A on the surface of RBCs, and the VIID11 variant of paraoxonase 1 (scFv-PON1). We show that scFv-PON1 variants expressed by Trichoplusia ni la... More