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PlexinD1 is required for proper patterning of the periocular vascular network and for the establishment of corneal avascularity during avian ocular development.

Dev Biol.. 2016-03; 
Kwiatkowski SC, Ojeda AF, Lwigale PY.
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Molecular Biology Tools ... Generation of RCAS-shRNA constructs. Using free online tools (Invitrogen, Dharmacon, Genscript), PlexinD1-specific sequences having 21 base pairs, GC content ranging between 30–50%, and beginning with a guanine residue were selected as PlexinD1-shRNA sequences. ... Get A Quote

摘要

The anterior eye is comprised of an avascular cornea surrounded by a dense periocular vascular network and therefore serves as an excellent model for angiogenesis. Although signaling through PlexinD1 underlies various vascular patterning events during embryonic development, its role during the formation of the periocular vascular network is yet to be determined. Our recent study showed that PlexinD1 mRNA is expressed by periocular angioblasts and blood vessels during ocular vasculogenesis in patterns that suggest its involvement with Sema3 ligands that are concurrently expressed in the anterior eye. In this study, we used in vivo knockdown experiments to determine the role of PlexinD1 during vascular patterning... More

关键词

Angiogenesis; Cornea; Ocular development; Periocular vascular plexus; PlexinD1; Vasculogenesis