至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Constitutive expression of the active fragment of human vasostatin Vs30 in Pichia pastoris SMD1168H

Protein Expr Purif.. 2018-04; 
Calderon-Salais S, Velazquez-Bernardino P, Balderas-Hernandez VE, Barba-de la Rosa AP, De Leon-Rodriguez A.
Products/Services Used Details Operation
Gene Synthesis … The routine chemicals were analytical grade (Sigma). 2.2. Construction of multi-copy expression vector. The vs30 sequence was optimized in accordance with the preferential codon usage for P. pastoris using the OptimumGene™ software and synthesized by Genscript™ … Get A Quote
Bacterial Expression System Get A Quote

摘要

Vasostatin 30 (Vs30) is an active fragment derived from the N-terminal region (135-164aa) of human calreticulin (hCALR) and has the ability to inhibit angiogenesis. In this work, the expression of Vs30 was performed using a protease-deficient strain of the methylotrophic yeast Pichia pastoris. The vs30 gene was optimized for P. pastoris preferential codon usage and inserted into constitutive expression vector pGAPZαA. In addition, a multi-copy strain was obtained using an in vitro multimerization approach. The flask fermentation conditions were: culture volume of 25 mL in 250 mL baffled flasks at 28 °C, pH 6 and harvest time of 48 h. Up to 21.07 mg/L Vs30 were attained and purified by ultrafiltration with a 3... More

关键词

Angiogenesis; Constitutive expression; Multi-copy strain; Spheroids; Vasostatin