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Primacy of cardiac chymase over angiotensin converting enzyme as an angiotensin-(1–12) metabolizing enzyme.

Biochem Biophys Res Commun.. 2016-09; 
Ahmad S, Varagic J, VonCannon JL, Groban L, Collawn JF, Dell'Italia LJ, Ferrario CM.
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Catalog Products ... 2. Materials and methods. 2.1. Reagents. Rat angiotensin-(1–12) (>99% purity) was purchased from GenScript USA Inc. (Piscataway, NJ). MLN-4760 (ACE2 inhibitor) was obtained from Millennium Pharmaceuticals (Cambridge, MA). ... Get A Quote

摘要

We showed previously that rat angiotensin-(1-12) [Ang-(1-12)] is metabolized by chymase and angiotensin converting enzyme (ACE) to generate Angiotensin II (Ang II). Here, we investigated the affinity of cardiac chymase and ACE enzymes for Ang-(1-12) and Angiotensin I (Ang I) substrates. Native plasma membranes (PMs) isolated from heart and lung tissues of adult spontaneously hypertensive rats (SHR) were incubated with radiolabeled (125)I-Ang-(1-12) or (125)I-Ang I, in the absence or presence of a chymase or ACE inhibitor (chymostatin and lisinopril, respectively). Products were quantitated by HPLC connected to an in-line flow-through gamma detector. The rate of (125)I-Ang II formation from (125)I-Ang-(1-12) by ... More

关键词

Angiotensin I; Angiotensin II; Angiotensin-(1-12); Angiotensin-converting enzyme; Rat cardiac chymase; Renin-angiotensin system