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Hyaluronan Depolymerization by Megakaryocyte Hyaluronidase-2 Is Required for Thrombopoiesis.

Am J Pathol.. 2016-09; 
Petrey AC, Obery DR, Kessler SP, Flamion B, de la Motte CA.
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Catalog Products ... After 24 hours, the cells were transfected with 0.5 μg of pGS-gRNA-Cas9 with a puromycin resistance cassette containing the Hyal-2 genomic RNA targeting sequence (ATACAGGCCTAGACGGTCG) (Genscript, Nanjing, China). ... Get A Quote

摘要

Hyaluronan is the predominant glycosaminoglycan component of the extracellular matrix with an emerging role in hematopoiesis. Modulation of hyaluronan polymer size is responsible for its control over cellular functions, and the balance of hyaluronan synthesis and degradation determines its molecular size. Although two active somatic hyaluronidases are expressed in mammals, only deficiency in hyaluronidase-2 (Hyal-2) results in thrombocytopenia of unknown mechanism. Our results reveal that Hyal-2 knockout mice accumulate hyaluronan within their bone marrow and within megakaryocytes, the cells responsible for platelet generation. Proplatelet formation by Hyal-2 knockout megakaryocytes was disrupted because of abn... More

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