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ECM turnover-stimulated gene delivery through collagen-mimetic peptide-plasmid integration in collagen.

Acta Biomater.. 2017-10; 
Urello MA, Kiick KL, Sullivan MO.
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Catalog Products ... Growth factor reduced (GFR) phenol red free Matrigel® was purchased from Corning (Corning, NY), BMP-2 was purchased from GenScript (Piscataway, NJ), and coelenterazine was purchased from Clontech and GoldBio (Yorba Linda, CA). 2.2. ... Get A Quote

摘要

Gene therapies have great potential in regenerative medicine; however, clinical translation has been inhibited by low stability and limited transfection efficiencies. Herein, we incorporate collagen-mimetic peptide (CMP)-linked polyplexes in collagen scaffolds to increase DNA stability by up to 400% and enable tailorable in vivo transgene expression at 100-fold higher levels and 10-fold longer time periods. These improvements were directly linked to a sustained interaction between collagen and polyplexes that persisted during cellular remodeling, polyplex uptake, and intracellular trafficking. Specifically, incorporation of CMPs into polyethylenimine (PEI) polyplexes preserved serum-exposed polyplex-collagen ac... More

关键词

Collagen; Collagen-like peptides; Collagen-mimetic peptides; Non-viral gene delivery