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Identification of Structure-Stabilizing Interactions in Enzymes: A Novel Mechanism to Impact Enzyme Activity.

Cell Biochem Biophys.. 2017-07; 
Serrano M, Gonzalez V, Ray S, Chavez MD, Narayan M.
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Mutagenesis Services ... Technologies). PCZN-WT-pQE30 was then subjected to mutagenesis to generate mutants: E25V; D82V; E87V; C141A by Genscript, USA. See Supplementary Fig. 1A–B for pQE30-PCZN-WT and mutants map constructs. Resi ... Get A Quote

摘要

Cruzain, a cysteine protease in the cathepsin family, is pivotal to the life-cycle of Trypanosoma cruzi, the etiological agent in Chagas disease. Current inhibitors of cruzain suffer from drawbacks involving gastrointestinal and neurological side effects and as a result have spurred the search for alternative anti-trypanocidals. Through sequence alignment studies and intra-residue interaction analysis of the pro-protein of cruzain (pro-cruzain), we have identified a host of non-active site residues that are conserved among the cathepsins. We hypothesize that these conserved amino acids play a critical role in structure-stabilizing interactions among the cathepsins and are therefore crucial for eventually gainin... More

关键词

Auto-activation; Chagas disease; Circular dichroism; Cysteine protease; Expression; Pro-cruzain; Trypanosoma cruzi