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1H, 15N and 13C resonance assignments of Ixolaris, a tissue factor pathway inhibitor from the tick salivary gland.

Biomol NMR Assign.. 2017-10;

De Paula VS, Silva FHS, Francischetti IMB, Monteiro RQ, Valente AP.

Products/Services Used	Details	Operation
PCR Cloning and Subcloning	... Methods and experiments The Ixolaris protein (corresponding to residues 1–140) was cloned into the expression vector pET-32a into the 5′ NcoI and 3′ BamHI restriction sites (Genscript). To avoid frame shift was added two nucleotides right after NcoI site (CCA TGG GT). ...	Get A Quote
Gene Synthesis		Get A Quote

摘要

Ixolaris is a two-Kunitz tick salivary gland protein identified in Ixodes scapularis that presents sequence homology to TFPI (tissue factor pathway inhibitor). It binds to the coagulation enzyme factor Xa (FXa) or to its zymogen form, FX, and further inhibits tissue factor/FVIIa complex (extrinsic Xnase compex). Differently from TFPI, Ixolaris does not bind to the active site cleft of FXa. Instead, complex formation is mediated by the FXa heparin-binding exosite, which may also results in decreased FXa activity into the prothrombinase complex. The Ixolaris-FXa/FX complex formation has been characterized by using a combination of biophysical and biochemical technics although no structural data is currently avail... More