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Autophagy mediates epithelial cancer chemoresistance by reducing p62/SQSTM1 accumulation.

PLoS ONE. 2018; 
BattistaR Alessia,ResnatiMassimo,FacchiCecilia,RuggieriElena,CremascoFloriana,ParadisoFrancesca,OrfanelliUgo,GiordanoLeone,BussiMario,CenciSimone,MilanEn
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ORF cDNA Clones/MolecularCloud … Saint Louis, MO). Human FLAG-p62 cDNA was purchased from Genscript (Piscataway, NJ) and cloned in a plasmid with a bidirectional human PGK promoter co-expressing p62 and truncated human LNGFR. FLAG-G263X … Get A Quote

摘要

To cope with intrinsic and environmental stress, cancer cells rely on adaptive pathways more than non-transformed counterparts. Such non-oncogene addiction offers new therapeutic targets and strategies to overcome chemoresistance. In an attempt to study the role of adaptive pathways in acquired drug resistance in carcinoma cells, we devised a model of in vitro conditioning to three standard chemotherapeutic agents, cisplatin, 5-fluorouracil, and docetaxel, from the epithelial cancer cell line, HEp-2, and investigated the mechanisms underlying reduced drug sensitivity. We found that triple-resistant cells suffered from higher levels of oxidative stress, and showed heightened anti-stress respons... More

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