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Molecular Biology Reagents> | … Materials and Methods. Reagents. The hollow fiber bioreactor was from Fiber Cell Systems Inc. (MD); Dulbecco's modified Eagle's medium (DMEM) was from Gibco or Sigma; penicillin- streptomycin was from Gibco; rSVmab was produced by Genscript Co., according to the … | Get A Quote |
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The voltage-gated Na channel subtype Nav1.7 is important for pain and itch in rodents and humans. We previously showed that a Nav1.7-targeting monoclonal antibody (SVmab) reduces Na currents and pain and itch responses in mice. Here, we investigated whether recombinant SVmab (rSVmab) binds to and blocks Nav1.7 similar to SVmab. ELISA tests revealed that SVmab was capable of binding to Nav1.7-expressing HEK293 cells, mouse DRG neurons, human nerve tissue, and the voltage-sensor domain II of Nav1.7. In contrast, rSVmab showed no or weak binding to Nav1.7 in these tests. Patch-clamp recordings showed that SVmab, but not rSVmab, markedly inhibited Na currents in Nav1.7-expressing HEK293 cells. Notably... More