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Development of the multi-epitope chimeric antigen rqTSA-25 from Taenia saginata for serological diagnosis of bovine cysticercosis.

PLoS Negl Trop Dis. 2018-04; 
Guimarães-PeixotoRafaella P M,PintoPaulo S A,SantosMarcus R,ZilchTiago J,ApolinárioPaula F,Silva-JúniorAbel
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Gene Synthesis … The resulting data converged in the epitope described below (Table 1), which was synthesized by Genscript (Piscataway, NJ, USA) with > 95% purity … The gene was synthesized by Genscript and the synthetic genes were cloned into the pET29a(+) expression vector … Get A Quote
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摘要

Bovine cysticercosis is a worldwide distributed zoonosis caused by the larval form of Taenia saginata present in bovine muscles. The diagnosis is based on the postmortem inspection at slaughterhouses and consists of the macroscopic visualization of lesions caused by cysticercosis in muscle sites. However, parasitized animals can pass unnoticed during sanitary inspection. Thus, the objective of this study was to characterize and evaluate the performance of different peptides from different regions of T. saginata for the cysticercosis diagnosis using enzyme-linked immunosorbent assay. We generated and evaluated a new recombinant protein chimera derived from the fusion of different peptides. We selected three ... More

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